Xenbase Image ID: 82354

Fig. S1. Expression of haematopoietic and endothelial genes in DLP adult haemangioblasts. (A) In situ hybridisation showing ETS TFs not expressed in the DLP. Black arrows indicate the position of the DLP. All embryos were hybridised as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. All embryos are shown at stage 26. (B) In situ hybridisation analysis showing the expression of blood and endothelial genes not expressed in adult haemangioblasts (Fig. 1) but expressed in developing blood vessels or developing blood cells. All embryos were hybridised as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. All embryos are shown at stage 34. (C) Expression analysis in sectioned control and Etv6 morphants showing co-expression of Lmo2 and VegfA in the DLP. In control embryos, dark blue staining (VegfA) masks the turquoise staining (Lmo2) in the DLP (red arrow) where both transcripts are expressed. By contrast, in Etv6 morphants, VegfA is no longer expressed in the DLP and therefore Lmo2 staining can be visualised (red arrow). Sections are in transverse orientation with dorsal to the top. Sections are taken from stage 26 embryos. DA, dorsal aorta; Hyp, hypochord; Lpm, lateral plate mesoderm; n, notochord; s, somites; Thromb, thrombocytes; VitV, vitelline vessels. Image published in: Ciau-Uitz A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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