XB-IMG-82355
Xenbase Image ID: 82355
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Fig. S2. SCL is required for embryonic erythrocyte differentiation. (A) Diagram showing the sequence targeted by the antisense MO (red nucleotides) blocking the translation of the two X. laevis SCL alleles. Initiation ATG is in purple. Sequences targeted by Scl MO2 and Scl MO3 are also indicated. (B) Western blot showing depletion of SCL protein in embryos injected with SCL MO at the two-cell stage. 2xHA-SCL, present only in stage 10 sample, indicates HA-tagged protein generated from exogenous mRNA. Note that no endogenous SCL protein is detected at stage 10. ACTIN was used as a loading control. (C) In situ hybridisation analysis showing the absence of embryonic erythrocytes, as indicated by expression of embryonic Globin and Gfi1b (arrows), in SCL morphants. Embryos were hybridised as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. All embryos are shown at stage 34. Numbers of embryos represented by each panel, out of the number analysed, are indicated in the top right corner. Image published in: Ciau-Uitz A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |