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Fig. S5. Adult haemangioblast programming in Fli1 morphants is not rescued by exogenous Gata2 mRNA. Fli1 MO was co- injected with a hormone-inducible form of Gata2, Gata2FL-GR-AH, at the two-cell stage and grown to stage 20 when Gata2FL-GR- HA activity was induced with 10 mM dexamethasone (Dex). Embryos were then further cultured and collected at the stages indicated. Expression of Flk1 at stage 22, Etv2 at stage 24 and Scl at stage 26 was not rescued in the DLP (arrows). Nevertheless, Scl expression in developing erythrocytes in the ventral blood island was significantly rescued (red arrowheads). Black arrowheads indicate Etv2 ectopic expression in the somites. Embryos were hybridised as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. Numbers of embryos represented by each panel, out of the number analysed, are indicated in the top right corner. Image published in: Ciau-Uitz A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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