Xenbase Image ID: 82429

Fig. 3. Mesoderm formation is inhibited by microinjection of Xnr5-KDEL mRNA. (A-E) Inhibition of mesoderm formation by using microinjection of Xnr5-KDEL mRNA (4 or 16 pg), as demonstrated by monitoring the presence of defects in the blastopore, as well as by using in situ hybridization analysis for the pan-mesodermal marker Xbra in the embryos at stage 11 of embryogenesis. (F-I) Resultant Cer-S mRNA-mediated phenotypic effects on the embryos that fail to develop mesoderm (shown in I) were also confirmed in embryos at later stages, as shown in (G,H). (J) The expression of all Nodal target markers such as Mix1, Mixer, Sox17, and Xbra was blocked following microinjection of Xnr5-KDEL mRNA. EF1a was used as a positive control. Total mRNA was prepared from the embryos at stage 9 of embryogenesis. Image published in: Matsukawa S et al. (2012) Copyright © 2012. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 mesoderm Image source: Published Larger Image Printer Friendly View

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