XB-IMG-82458
Xenbase Image ID: 82458
|
Figure 4. Comparison of ion channel and pump gene expression in chick and Xenopus. We compared the expression of the same ion channel clones from chick and Xenopus by in situ hybridization. A: The K+ channel xKv2.2 was expressed very strongly in the organizer during Xenopus gastrulation (arrow). B,C: Sectioning reveals staining deep in organizer cells (arrows). Similarly, in chick, cKv2.2 was expressed in the base of the nascent primitive streak at stage (st.) 1 (arrow, D) and in the streak itself as it elongates (arrows, E,F). The potassium channel K(v)LQT-1 is also expressed in the primitive streak in the chick (arrow, G) and then in most tissues at st. 8 (arrow, H). I: In contrast, in Xenopus, we detect no expression by in situ hybridization at gastrulation stages (data not shown), but at neurulation, it is expressed in a horse-shoe pattern very similar to the location of the neural crest (arrows). Maternal mRNA for K(v)LQT-1 is located in the animal halves of cells at early cleavage stages (J, showing the inside surface of half of a four-cell embryo manually split down the cleavage plane after in situ hybridization; Ja: section of 1-cell embryo, Jb: inside surface of wholemount 2-cell embryo split down the cleavage plane). K: In chick embryos, the 16-kDa proteolipid subunit of the vacuolar ATPase is expressed in the head-folds of the closing neural tube (red arrows); it can also be seen in the regressing primitive streak (yellow arrow). In Xenopus, maternal mRNA for the 16-kDa proteolipid subunit can be detected as maternal mRNA in animal cells during cleavage (arrows, L), and similarly to the chick, is localized to the neural tissues in the dorsal aspect of the embryo at somite stages (arrows, M). Red arrows indicate regions of expression. Image published in: Rutenberg J et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons.
Image source: Published Larger Image Printer Friendly View |