Xenbase Image ID: 82505

Figure 5 Anterior somite disruption. Embryos were unilaterally injected with mRNA coding for nuc-LacZ together with control morpholino oligo (CMO) or antisense morpholino oligos targeted against MyoD (MyoD AMO). The myocyte nuclei align down the center of each somite in normal embryos (100%, n=14). Disrupted somites are evident by the failure of these nuclei to align (arrows). This disruption is most evident in somites 4-7 in MyoD knock-down embryos (58%, n=12) while more posterior somites in these same embryos form normally. Somites form in an anterior-to-posterior direction, so this suggests that the effects of MyoD knockdown on somite morphogenesis is transient; this is consistent with the recovery of segmentation genes like Tbx6 and mesogenin at neurula stages. Image published in: Maguire RJ et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + myod1 MO + NF35/36 (immunohistochemistry) fig.5 Anatomical Phenotype abnormal nucleus morphology abnormal somite morphology abnormally irregular spatial pattern of somite Larger Image Printer Friendly View

Return to previous page