XB-IMG-82506
Xenbase Image ID: 82506
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Figure 4.
Endogenous localization of let-7a and miR-18a in early vertebrate embryos. (A) In situ hybridization showing endogenous let-7a and miR-18a in E7 mouse embryos viewed in whole-mount (A,D) or transverse histological section (B,E). (C,F) High-magnification view distinguishing epiblast mesoderm and endoderm layers. Note the abundant expression of let-7a (A) and miR-18a (D) in ectoderm and mesoderm but not in endoderm. (G) Endogenous Xlet-7a in cleavage stage Xenopus embryos showing expression in the animal hemisphere (presumptive ectoderm and mesoderm). (S) Endogenous Xlet-7a expression in gastrula stage (stage 10.5) Xenopus embryos. View of the mesoderm in the equatorial region marked by Xbra (S) and involuting endoderm marked by Xsox17α (T) reveals overlap with Xlet-7a prominently in mesoderm (U). (V) Transverse sections show expression domains of Xbra (V), XleftyA (W), and Xsox17α (X) relative to Xlet-7a (Y), revealing that the miR is present in both ectoderm and mesoderm but not in endoderm and is nonoverlapping with XleftyA but coincident with Xacvr1b (Z) mRNAs. Image published in: Colas AR et al. (2012) Copyright © 2012. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |