XB-IMG-82512
Xenbase Image ID: 82512
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Figure 6.
Blocking endogenous let-7 converts ectoderm to endoderm in embryos. (A) Embryos at the 16-cell stage were injected in one animal blastomere (presumptive ectoderm) together with Xlet-7 TP or control morpholino and LacZ mRNA dextran as a lineage label. Whereas the progeny of control morpholino-injected blastomeres contributed exclusively to epidermis (A), Xlet-7 TP morpholino-injected blastomeres contributed to endoderm, examined at the tailbud stage (stage 35) in lateral view (A,D), transverse bisection (B,F), dorsal view (C,G), and ventral view (D,H). (I) The same as in A, but examined at the tadpole stage (stage 45) and injected with Alexa Fluor546 dextran as a lineage label. Note the pronounced diversion of ectoderm to gut cell fate conversion, viewed ventrally. Image published in: Colas AR et al. (2012) Copyright © 2012. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |