Xenbase Image ID: 82514

Figure 1. The Structure and Stability of SPG-Generated mRNAs In- jected into Xenopus Embryos (A) Diagramatic representation of the SP6 mRNAs used in injection experiments. 64T-CIA encodes the complete Xhox-1A protein. 64T- clAABgl2 encodes the same protein except that 13 C-terminal amino acids of the homeobox and the remaining 32 C-terminal amino acid of the protein are deleted. A 23 amino acid extension to the homeobox has been created from a reading frame other than that specifying Xhox-1A. XBm codes for Xenopus 8-globin. Details of construction of the three RNAs are found in Harvey et al. (1986). The open box is the coding region of the Xhox-IA mRNAs, the cross-hatched box is the globin coding region, and the striped box is the homeobox. The elipse at the 5nd of each mRNA indicates that a 5ap has been added during synthesis (Krieg and Melton, 1988). (B) Northern blot analysis of RNA from embryos injected with either 64T-clA or XBm RNA extracted at different stages of development. The injected RNAs were detected on Northern filters with nick-translated Xhox-IA or globin DNA probes. Stages are those specified by Nieuwkoop and Faber (1956). (C) Quantitation of the results seen for 64T-CIA in (8). Quantities of in- jected RNA were determined relative to known amounts of the syn- thetic RNA run on the same gel. The endogenous levels of the Xhox-1A mRNA were previously measured (Harvey et al., 1986). Note that the scale for injected and endogenous RNAs are in ng and pg, respec- tively. Image published in: Harvey RP and Melton DA (1988) Copyright © 1988. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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