Xenbase Image ID: 82519

Figure 6. lmmunohistochemrstry on a Xhox-1A Injected Embryo Usrng the Muscle-Specific Antibody 12/101 (A) Horizontal section of a Xhox-1A injected embryo stained with DAPI nuclear stain. Note the aligned nuclei in each normal somite in the upper, umnjected side of the embryo, and in the posterior region of the lower, injected side. Nuclei in the disturbed anterior portion of the lower injected side are not aligned. (6) The same section as in (A) stained by indirect immunofluorescence with the muscle-specific monoclonal antibody f2/tOt. This antibody highlights the membranes of myocytes. (C) and (D) 2.5x enlargements of different parts of the immunofluorescence shown in (B). The arrow in (C) indicates tissue not stained by the antibody. The arrow in (D) indicates a patch of myocytas in cross section surrounded by cells elongated in the plane of the tissue section. B is brain, E is eye, EV is ear vesicle, NC is notochord, and S is somitic mesoderm. Image published in: Harvey RP and Melton DA (1988) Copyright © 1988. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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