Xenbase Image ID: 82520

Figure 7. Localization of the Endogenous Xhox-IA Transcript. Embryos were separated into the sections indicated, total RNA was extracted, and then analyzed for the presence of endogenous Xhox-IA mRNA by RNAase protection. Probes for EFla (a translation elongation factor; see Kintner and Melton, 1987) and cytoskeletal actin were used to control for RNA recovery. Similarly, a probe for muscle-specific actin was used to control for contamination of sections with muscle. (a) Examination of Xhox- 1A localization in the principal axes of the early embryo. A is anterior, P is posterior, NP is the neural plate (containing both the neural ectoderm and the underlying mesoderm), H is the head, 0 is dorsal, and V is ventral. Note that Same muscle contaminates the R, H, and V samples. Higher resolution dissections were performed in (b). (b) Dissections to separate the axial structures from the lateral mesoderm and endoderm. T is the total embryo, H is the head, Ax is the dorsal axial structures, En is the endoderm, and LP is the lateral plate mesoderm and ventral ectoderm. The poor recovery of RNA in the endoderm sample is due to these cells being large and filled with yolk granules. They contain less cytoplasm and RNA. Image published in: Harvey RP and Melton DA (1988) Copyright © 1988. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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