XB-IMG-82540
Xenbase Image ID: 82540
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Fig. 5. Expression of human and mouse LIN-28 in cultured cells, embryos,
and tissues. (A) Immunoblot of cultured mouse and human cell lines using
antiserum raised against full-length recombinant human LIN-28 protein.
ES fb, mouse embryonic stem cells with mouse fibroblast feeder cells; fb,
mouse fibroblasts alone; MRC-5, human fetal lung fibroblast; HeLa, human
cervical adenocarcinoma; HL-60, human promyelocytic leukemia;
MCF7, human breast adenocarcinoma; A2780, human ovarian adenocarcinoma;
NT2, human embryonal carcinoma; NT2N, neurons from retinoic
acid-treated NT2 cells. Immunoblot using anti-LIN-28 antisera of cultured
cells, embryos and tissues. F9, mouse embryonal carcinoma; P19, mouse
embryonal carcinoma; PA-1, human teratocarcinoma; PC-12, rat adrenal
pheochromocytoma; 9.5d, 10.5d, 12.5d, mouse embryos at 9.5, 10.5, and
12.5 days postcoitum, respectively; neuron, mouse primary hippocampal
neurons; liver, liver from 3- to 4-month-old mice. All samples were
normalized for total protein content. Anti-actin was used as a loading
control. (B) Micrographs of mouse P19 teratocarcinoma cells stained with
anti-LIN-28 antiserum. Left, fluorescence micrograph showing primarily
cytoplasmic staining. Right, a DIC image of the same field. Image published in: Moss EG and Tang L (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |