Xenbase Image ID: 82708

Fig 3. Southern blot analysis of transgenic lines. A: Schematic diagram of pElastase-GFP-SceI containing a pancreas-specific promoter and a green fluorescent protein (GFP) coding region. B: Southern blot analysis was performed with genomic DNA isolated from F1 offspring of four F0 founder frogs (lines 2-5) injected with pElastase-GFP-SceI and scored positive for GFP in the pancreas. The a and b indicate two different individuals from the same line. Genomic DNA was digested with BamHI and hybridized with insert probes resulting from NotI digestion. The copy number of integrated DNA was estimated using a dilution series (as indicated) of plasmid DNA digested with BamHI. 2X-64X, plasmid standards. Quantities indicated on top of each lane correspond to 2 to 64 copies of the plasmid integrated into the genome. Image published in: Pan FC et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page