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Xenbase Image ID: 82784

Figure 4. Activation of BMP or Notch Signaling Pathways during the Refractory Stages Promotes Regeneration of Tail TissuesNontransgenic . (A) and transgenic tadpole tails (C-E) shown 7 days after removal of 50% of the tail at stage 47. All tadpoles received a heat shock 3-4 hr before amputation and subsequent daily heat shocks. (A) Wild-type (WT) tadpoles as controls for transgenics. No regeneration of tail tissues from the stump has occurred. (B) Tadpoles transgenic for the HSP70-Alk3-γCrys-GFP construct can regenerate their tails completely. (C) Tadpoles transgenic for the HSP70-NICD-γCrys-GFP construct partially regenerate their tails, reforming the spinal cord and notochord. (D) Tadpoles transgenic for the HSP70-eveMsx1-γCrys-GFP construct can regenerate their tails completely. (E) Replacing eveMsx1 with the nonfunctional, N-terminal-deleted δNMsx1 in the transgene cassette abolishes regeneration ability. White arrows show the level of amputation. Tails are shown in lateral view, anterior to the left and dorsal uppermost. (F�H) Analysis of Msx1 expression (blue staining) in refractory stage tadpoles 2 days after amputation and heat shock. (F) Msx1 is expressed in HSP70-Alk3-γCrys-GFP transgenics. (G) Msx1 is not expressed in HSP70-NICD-γCrys-GFP transgenics. (H) Msx1 is not expressed in nontransgenic sibling controls. (I-N) Analysis of tissue composition of 7 day regenerates during the normal refractory period. Immunohistochemical staining (black/

Image published in: Beck CW et al. (2003)

Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.

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