Xenbase Image ID: 82831

Figure 5. Ngn3-GR promotes beta cell differentiation. (A) RT-PCR for insulin of whole embryos at Stages 24 and 26. (b,c) Increased pax4 expression detected in Dex treated embryos at Stage 32 (n = 15/17). (d,e) Increase in the mature beta cell marker, sur1, at Stage 32 (n = 19/31) in Dex treated embryos. In panel (d), arrow indicates nonendodermal expression of sur1. In panel (e), bracket denotes ectopic expression in endoderm. (f,g) Increase in somatostatin at Stage 32 (n = 12/27) in Dex treated embryos. In panel (F), arrow indicates expression of som outside the endoderm. In panel G, bracket denotes ectopic expression in endoderm. (h) RT-PCR at Stage 26 of whole endoderm (WE) and whole endoderm plus mesoderm explants (WEM). Explants were dissected at Stage 15 following 4 h of Ngn3-GR activation, cultured until Stage 26 and collected for RT-PCR. Twist and FoxF1 are mesoderm markers, which are detected only in the WEM explants. Image published in: Oropeza D and Horb M (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue pax4.L pax-4 X. laevis Throughout NF stage 32   abcc8.L abc36, hhf1, hrins, LOC108714951, phhi, sur, sur1, tndm2 X. laevis Throughout NF stage 32 somite sst.1.S smst, som, somatostatin X. laevis Throughout NF stage 32 mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + neurog3-GR + DEX + NF32 (in situ hybridization) Fig 5 c Anatomical Phenotype abnormal type B pancreatic cell differentiation Expression Phenotype increased amount pax4.L expression in dorsal pancreatic bud Xla Wt + neurog3-GR + DEX + NF32 (in situ hybridization) Fig 5 e Anatomical Phenotype abnormal type B pancreatic cell differentiation Expression Phenotype increased amount abcc8.L expression in dorsal pancreatic bud mislocalised abcc8.L expression in foregut endoderm Xla Wt + neurog3-GR + DEX + NF32 (in situ hybridization) Fig 5 g Anatomical Phenotype abnormal type B pancreatic cell differentiation Expression Phenotype mislocalised sst.1.L expression in foregut endoderm increased amount sst.1.L expression in somite decreased amount sst.1.L expression in somite Larger Image Printer Friendly View

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