XB-IMG-82860
Xenbase Image ID: 82860
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FIG. 1. Western blot analyses. Total embryo protein from stage 40
embryos was prepared and separated by SDS-polyacrylamide gel
electrophoresis and either stained with Coomassie brilliant blue (not
shown) or electrophoretically transferred to nitrocellulose paper and
probed with various antibodies. Each lane contains the proteins of
approximately four embryos. The anti-acetylated tubulin antibody
6-llB-1 (lane A) reacted with a single band (small arrow) of 55 kDa.
This band migrated slightly slower than P-tubulin, recognized by the
anti-P-tubulin antibody Ei� (lane B). The anti-NFm antibody RM044
reacted with a single band of approximately 175 kDa (lane C). The
positions of molecular weight markers (aa macroglobulin, 180 kDa;
j3-galactosidase, 116 kDa; bovine serum albumin, 66 kDa; ovalbulin, 45
kDa) are marked on the left side of lane A. Image published in: Chu DT and Klymkowsky MW (1989) Copyright © 1989. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |