XB-IMG-82861
Xenbase Image ID: 82861
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WC. 2. Microtubules in the egg. Whole-mount, immunoperoxidase stained eggs stained with anti-acetylated tubulin antibody (A) or
anti-beta-tubulin (B) reveal that the arrested meiotic spindles of the eggs contain acetylated tubulin. Staining of eggs with anti-beta-tubulin (C-E) at
various times after fertilization reveals the appearance and growth of the sperm aster (C, 15 min postfertilization; D, 20 min postfertilization;
E, 25 min postfertilization). In early stages of sperm aster growth, a distinctive structure is visable at the center of the growing aster (arrows in
C and D; the outer boundary of the sperm aster is marked by arrowheads in D and E). Staining of eggs with anti-acetylated tubulin 20-25 min
postfertilization revealed only a small structure (F, marked by arrow). At higher magnification (F�) this structure appears irregularly
spherical. Cortical immunofluorescence staining of the fertilized egg 75 min after fertilization (first cleavage at 90 min) was used to visualize
the cortical microtubule system (G- anti-/3-tubulin antibody/fluorescein-conjugated, anti-mouse immunoglobulin secondary antibody; rotation
direction marked by double-headed arrow). This cortical microtubule array was not stained significantly by anti-acetylated tubulin (H). Bar in
A marks 20 pm for A and B, bar in C marks 50 pm for C-F, bar in F� marks 10 pm; bar in G marks 10 Frn for G and H. Image published in: Chu DT and Klymkowsky MW (1989) Copyright © 1989. Image reproduced with permission of the Publisher, Elsevier B. V.
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