XB-IMG-82862
Xenbase Image ID: 82862
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FIG. 3. Microtubules in the blastula and gastrula stage embryo. Stage 7 (64112%cell) blastulas were stained with anti-acetylated tubulin
antibody (A-G, I) or anti-fi-tubulin (H). The anti-acetylated tubulin antibody labeled mitotic midbodies (A, C, arrows in A) and spindles (B, E).
The midbodies were striking in that they appear cylindrical, with an unstained core (C, D, D was taken from a 16-cell embryo). Spindles
appeared either rectangular (B, E) or rhombodal (G), with discrete polar regions (arrowheads). While interphase arrays were difficult to
visualize with anti-acetylated tubulin, the spindle poles of prophase cells could clearly be seen (F, marked by arrowheads). The interphase
array of microtubules could be seen in blastulas using anti-P-tubulin antibodies (H, i marks interphase cells, p marks prophase cell). During
late blastula/early gastrula stages, anti-acetylated tubulin stained both spindles and interphase microtubule arrays clearly (I, m marks
metaphase cell, p marks prophase cell, unmarked arrowhead points out cortical staining of blastomere). Staining of a gastrula stage embryo
with anti-fl-tubulin revealed the extensive subcortical microtubule system (J, subcortical microtubules marked by arrows). The difference in
the image in J, compared with H and I reflects focusing on the deeper cortical microtubule system. Bar in A marks 50 pm for A and B; bar in C
marks 10 pm for C-F; bar in H marks 50 pm for H-J. Image published in: Chu DT and Klymkowsky MW (1989) Copyright © 1989. Image reproduced with permission of the Publisher, Elsevier B. V.
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