XB-IMG-82867
Xenbase Image ID: 82867
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FIG. 8. Acetylated tubulin and NFm expression in the embryo. In a montage of parasagittal optical sections of a stage 32 embryo stained with
anti-acetylated tubulin (A) the ciliated epidermal cells can be clearly seen scattered over the embryo. The nervous system is also strongly
stained. In particular, cell bodies in the dorsal portion of the spinal cord can be easily resolved (arrow) along with fine processes extending into
the periphery (arrowheads). In B, a stage 32 embryo stained with anti-NFm reveals a similar pattern of staining within the neural tube (arrow
points to neuronal cell bodies in the dorsal region of the neural tube). Periphery motor axons can be clearly resolved (arrowheads). The pattern
of motoneurites in the periphery is diagrammed in C. In the tail/trunk region (marked TT) motor axons typically emerge from the neural tube
and run posteriorly toward the somite boundary. The positions of the somite boundaries were determined by examining the embryo under
polarization optics (see Fig. 9) and marked on the diagram with arrowheads and thin dashed lines at the somite boundaries. In the anterior
trunk region (marked AT), motor axons project both anteriorly and posteriorly. In the anterior-most region (marked Ant), motor axons project
primarily toward the anterior boundary of the somite. However, some motor axons clearly project caudally. The position of the eye, eye; otic
vesicle, OtV; and cement gland, cg, are marked in C. In B and C, a, b, and c correspond to the same regions shown in Fig. 9. Bar in B marks 100
pm for all parts. Image published in: Chu DT and Klymkowsky MW (1989) Copyright © 1989. Image reproduced with permission of the Publisher, Elsevier B. V.
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