Xenbase Image ID: 82897

Fig. 3. Expression of eye markers in X-DG morphants. Xotx2 (A, C, E, EV), Xrx1 (B, D, F, FV) and b-crystallin (G, GV) mRNA expression was detected by BMP purple (blue staining) in Mo-X-DG-injected embryos at stage 14 (A, B), 25 (C, D) and 28 (E�GV). Nuclear beta-gal staining (red) was used to trace the side of injection. Panels A�D show both injected and uninjected sides in frontal views. Panels E�GV show lateral view of uninjected (E, F, G) and injected (EV, FV, GV) sides, respectively. No changes in the expression domains of Xotx2 (A) and Xrx1 (B) are detectable between the two sides at stage 14. White brackets highlight the decrease of Xotx2 (C) and Xrx1 (D) expression in the eye of the injected sides at stage 25. Decrease is evident also at stage 28 (compare panel E to EV and F to FV); beta-crystallin expression is also dramatically reduced in the injected side (compare panel G to GV). Mo-X-DG injection completely abolishes also Xotx2 rostral expression domain in panels E to EV. Image published in: Lunardi A et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 14 optic field preplacodal ectoderm anterior placodal area pre-chordal neural plate otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 25 pre-chordal neural plate preplacodal ectoderm eye eye primordium optic vesicle otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 28 eye brain forebrain midbrain crybb1.S Beta-Crystallin-b1, catcn3 X. laevis Throughout NF stage 28 lens rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 14 pre-chordal neural plate optic field rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 25 eye optic vesicle rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 28 eye pineal gland retina Image source: Published Larger Image Printer Friendly View

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