XB-IMG-82900
Xenbase Image ID: 82900
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Fig. 6. Effects of the unilateral injection of Mo-X-DG (8 ng) at stage 4 cells on
the apoptosis and cell proliferation of the eye. (A�F) Eye sections of stage 32
embryos. Pe: pigmented epithelium. Detection of GFP, which was used as
tracer, is shown in panels B, D, F. Panels A�D show BrdU immunodetection
(red nuclei) in the control side (A, C) and Mo-injected side (Mo, B, D) of the
embryo. Hoechst counterstaining labels all nuclei in panels C, D. Panels E and
F show TUNEL detection of apoptotic nuclei (brown staining) in control (E)
and Mo-X-DG-injected side (F), respectively. Sections of Mo-X-DG injected
eyes always displayed a significantly higher number of TUNEL-positive cells
than sections of uninjected, contralateral eyes. Eyes injected with control-Mo
had the same number of TUNEL-positive cells than uninjected eyes (not
shown). The numbers of BrdU-positive nuclei in control and Mo-X-DGinjected
(Mo) retinas, expressed as percent over the total number of cells
analyzed (control: n = 550, Mo: n = 514), are compared in panel G. The
average number of TUNEL-positive cells per eye section (n = 120 sections) in
control and Mo-X-DG-injected retinas is compared in panel H. Bars indicate
SE in panel G and SEM in panel H. Differences between control and Mo are
significant in panel G (t test: P = 0.008904109) and in panel H (t test: P =
8.997E�06). Image published in: Lunardi A et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |