XB-IMG-82902
Xenbase Image ID: 82902
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FIG. 2. Pax-6 induces ectopic lenses in whole embryos in a cell autonomous fashion. b-Crystallin staining of embryos (stage 38) injected
with RNA-encoding Pax-6-FLAG (0.12� 0.25 ng/embryo) revealed the formation of ectopic lenses (brown) in whole mount (A�C) and in
section (D �G). Red arrowheads (B, F, and G) indicate the border between lens epithelium and fibre cells; large arrowhead in B indicates
endogenous lens. The embryo in A was cleared in benzyl benzoate and is at a higher magnification in B. Embryos shown in C and D were
costained (blue) with antibodies to NCAM. Some ectopic lenses were not associated with neural tissue (C, black arrowhead; D and E)
while others were close to (D, far left ectopic lens; F) or tightly associated with neural tissue (C, white arrowhead; G). Sections through
endogenous (H, left) and ectopic lenses (H, arrowheads, and I) in embryo coinjected with b-Gal and b-crystallin RNAs. Coincident staining
for b-Gal (blue) and b-crystallin (brown) indicates the cell-autonomous function of Pax-6. Magnifications (A) 150, (B, C) 1200, (D) 1100,
and (E� I) 1400. Image published in: Altmann CR et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |