Xenbase Image ID: 82903

FIG. 3. Neuralizing treatments result in the expression of Pax-6 and lens-specific bB1-crystallin in animal caps. Injection of RNA-encoding dominant-negative type I BMP receptor (lane 1, 2.0 ng), follistatin (lane 2, 2.0 ng), noggin (lane 3, 2.0 ng), chordin (lane 4, 2.0 ng), dissociated animal cap cells (lane 6), but not uninjected embryos (lane 5) led to the expression of NCAM, Pax-6, and bB1-crystallin in animal caps at tailbud stage. EF-1a was amplified as a loading control and the absence of muscle actin indicated that mesoderm was not present. RNA from tailbud embryo was used as a positive control while an amplification without RT (lane 7) controlled for the absence of contaminating DNA. Image published in: Altmann CR et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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