Xenbase Image ID: 82993

Fig. S1. Related to Fig. 1. Temporal and spatial expression pattern of itpkb. (A) itpkb quantitative (q)RT-PCR was done on embryos at different stages of development. Expression levels were normalized relative to rpl8. (B) qRT-PCR performed on animal caps at mid-blastula stage (stage 8) shows an enrichment of itpkb expression in cells fated to become epidermis. (C) Double fluorescent in situ hybridization for itpkb and sox3 combined with DAPI staining. MERGE represents nuclear (DAPI) staining in blue, itpkb in green, and sox3 in magenta. (Bars, 200 μm.) Image published in: Soto X et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page