Xenbase Image ID: 83064

Fig. 2. Expression of ZFNs targeting noggin in X. tropicalis. (A) Optimization of ZFN delivery in X. tropicalis. EL+KK: ZFNs with the EL and KK modifications in the FokI domain (8); numbers represent different noggin ZFN pairs (Table S1). WT, ZFNs with wild-type Fok1 nuclease domains. PA−, ZFN transcripts lacking a polyadenylation signal. UC, uninjected control. (B) Comparison of different noggin ZFN pairs in the yeast activity assay and in injected tadpoles. Tadpoles were injected with 100 pg ZFN mRNA. Yeast activity values are represented as a percentage relative to ZFNs targeting the human CCR5 gene (9). Activity in tadpoles is calculated as the percentage of mutant amplicons sequenced from injected embryos. ND, no data. (C) Western blot for FLAG-tagged ZFN proteins. Image published in: Young JJ et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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