XB-IMG-83123
Xenbase Image ID: 83123
|
|
Fig. 2. Temporal expression pattern of Pod 1 in X. laevis. RT-PCR was
performed on unfertilised Xenopus laevis eggs (UF) and whole Xenopus
laevis embryos at stages 9, 13, 16.5, 21, 26.5, 30 and 35, using Pod 1
primers. Equalisation was carried out using ODC as a loading control and
linearity was performed with doubling dilutions of input cDNA from stage
35 embryos to reduce the risk of a PCR plateau. Negative controls were
carried out as described in the methods. RT-PCR analysis shows that Pod
1 expression is not initiated until the beginning of neurulation, stage 13
followed by a gradual increase in expression until stage 35. Image published in: Simrick S et al. (2005) Copyright © 2005. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View |