XB-IMG-83124
Xenbase Image ID: 83124
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Fig. 3. Whole embryo and dissected kidney temporal expression of
Pod 1 in X. laevis. (A) Pronephric tissue or presumptive pronephric tissue
was dissected from Xenopus laevis embryos at stages 12.5, 15, 20, 28 and
35 (Brennan, et al., 1998) and RT-PCR was performed using specific Pod
1 primers. The RT-PCR was equalised using ODC and linearity and
negative controls carried out as described in Experimental Procedures.
Expression of Pod 1 in whole embryos (W) was compared with that of the
kidney or kidney primordium dissections (K). The whole embryo temporal
expression pattern confirms the profile shown in Fig 2. The Pod 1
expression in the kidney dissections, however, is proportionally greater
than that of the whole embryo, first appearing during neurulation, stage 15
and increasing as the embryo develops. (B) The pronephros from a
Xenopus laevis stage 42 embryo was dissected into the glomus, tubules and duct regions and RT-PCR using Pod 1 primers was carried out. Equalisation was carried out using EF1α and linearity and negative controls carried
out as described in Experimental Procedures. A fine dissection of the pronephros of a stage 42 X. laevis tadpole reveals the spatial expression of Pod1
within the pronephros. Pod 1 appears to be most expressed in the glomus, less in the tubules and weakest in the duct. Image published in: Simrick S et al. (2005) Copyright © 2005. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View |