Xenbase Image ID: 83163

Figure 5. Dzip1 regulates the stability of Spop, independent of its role in ciliogenesis. (A) Western blot showing that the stability of Spop, but not that of Sufu, was decreased in Dzip1 depleted embryos. RNAs encoding myc-tagged (MT)-Spop (250 pg) or MT-Sufu (250 pg) were co-injected with MT-GFP (50 pg) into controls or embryos that had received a prior injection of DMO or IFT88 MO. Embryos were harvested at the neurula stage. MT-GFP serves as injection and loading control. (B) Immunofluorescence showing a significant reduction of motile cilia on the epidermis (top panel) and primary cilia in the floor plate (bottome panel) within embryos injected with 40 ng IFT88 MO (right column), compared to controls (left column). (C) DMO Image published in: Schwend T et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 35 and 36 epidermis spinal cord floor plate ciliated epidermal cell Image source: Published Larger Image Printer Friendly View

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