Xenbase Image ID: 83216

Noggin mutant proteins synthesized by Xenopus oocytes. Western blot analysis of Xenopus oocytes that were injected with capped RNA from pT7TS constructs containing SYM1-derived sequences (P223L and G189C) and SYNS1-derived sequence (W217G). Samples were subjected to nonreducing SDS/12% PAGE and immunodetected using an anti-noggin antibody. All noggin mutants form disulfide-bonded dimers, albeit with varying efficiency. Monomeric noggin migrates at 32 kDa, disulfide-bonded dimeric noggin migrates at 52 kDa, and a high molecular weight noggin aggregate (denoted by *) does not penetrate the separating gel. Image published in: Marcelino J et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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