XB-IMG-83324
Xenbase Image ID: 83324
Fig. 9. Dazap2 activity does not depend on canonical WNT signaling. (A) RT-PCR was performed using whole embryos injected with fgf8a (100 pg/cell) either alone or in combination with β-catenin MO (either 40 ng/cell or 60 ng/cell) into two cells at the 2-cell stage, animal caps were dissected and then aged until stage 19 (WE, whole embryo sibling controls; -RT, no reverse transcriptase control; Ni, non-injected explant control). Hoxb9 expression is lost at both concentrations of β-catenin MO (lanes 5 and 6) when compared to Fgf8a alone (lane 4). (B) When the experiment is repeated using Dazap2 (125 pg/cell), β-catenin MO is unable to block Dazap2 induction of HoxB9 expression (compare lane 4, with co-injection lanes 5 and 6) Image published in: Roche DD et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |