Xenbase Image ID: 83412

Fig. 3. Gnas is the target of cholera toxin action. (A) Schematic of signaling via the Gαs G-protein family. The activity of Ctx is indicated in red. (B and C‴) In situ hybridization of Xenopus embryos at stage 35 for Gnas and Gnal mRNA by whole mount or paraplast sections thereof. The position of the transverse sections is indicated in (B and C). (D�Q) Whole mount immunostaining with the 3G8 (D�I) and 4A6 antibody (K�P) in control embryos or embryos injected with the Gnas-MO or the Gnal-MO in the presence or absence of 2 μg/ml Ctx at stage 40. Ectopic 4A6 staining is indicated by red arrowheads. Quantification of proximal tubular cell numbers at stage 42 (J) and ectopic 4A6 staining at stage 40 (Q) summarizing three independent experiments. Data were analyzed by Student's t-test with indicating a p value of <0.01. Image published in: Zhang B et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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