Xenbase Image ID: 83414

Fig. 5. Cholera toxin regulates albumin uptake and secretion. (A-B‴) Untreated or Ctx-treated Xenopus embryos were injected with Albumin-FITC (green) at stage 35. Embryos were fixed after 3 h, stained with 3G8 (red) and examined in whole mounts (A and A″, B and B″) and sections thereof (A‴ and B‴). DAPI was used to counterstain nuclei (blue). (C) Schematic for the uptake assay in HK-2 cells. (D) Bar diagram of Albumin-FITC uptake of HK-2 cells treated with different doses of Ctx, cAMP-Epac, cAMP-PKA and BFA. (E) Schematic for the secretion assay in HK-2 cells. (F) Bar diagram of Albumin-FITC secretion of HK-2 cells treated with different doses of Ctx, cAMP-Epac, cAMP-PKA and BFA. Data were analyzed by Student's t-test with indicating a p value of <0.05 and a p value of <0.01. Image published in: Zhang B et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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