Xenbase Image ID: 83418

Supplementary Fig. S3. Efficacy of the antisense morpholino oligomers. (A) Schematic of the GFP fusion proteins containing the 5′UTR and part of the coding sequence of Gnas and Gnal. The position of the Gnas-MO and Gnal-MO are indicated. (B�G) Xenopus embryos were injected animally with the Gnas-MO and Gnal-MO at the 2�4 cells stage followed by two diametral injections of synthetic mRNA encoding either Gnas- or Gnal-GFP at the 8-cell stage, while control embryos were only injected with the Gnas- or Gnal-GFP mRNA. Embryos were analyzed at stage 10.5 by florescence microscopy. Representative images are shown and the percentage of GFP-positive embryos is indicated in the upper right corner of each panel. (H) Schematic of the exonic sequence targeted by Rapgef4-sMO. Positions of the PCR primers used to test the splicing efficacy are indicated. (I) Representative image of the PCR reaction using mRNA from embryos injected with Rapgef4-sMO or uninjected controls. Position of the correctly spliced and the unspliced mRNA are indicated by one or two asterisks, respectively. Image published in: Zhang B et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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