XB-IMG-83457
Xenbase Image ID: 83457
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Fig. 3. XTRAP-γ MO affects pronephric tubulogenesis. (A) XTRAP-γ MO
specificity in vitro. Western-blotting analysis showed significant inhibition of
XTRAP-γ- HA (100 pg/embryo) protein synthesis by XTRAP-γ MO (40 ng/embryo).
XTRAP-γ- HA (100 pg/embryo) protein synthesis was not affected with 5-mis-
XTRAP-γ MO (40 ng/embryo). (B-G) Whole-mount immunohistochemistry analysis
on pronephros. (B) XTRAP-γ MO (40 ng), (D) 5-mis-XTRAP-γ MO (40 ng) and
(E) XTRAP-γ MO (40 ng) plus 5-mis-XTRAP-γ mRNA (1 ng) were injected into one
side of the lateral marginal zone of 16-cell-stage embryos. Embryos were fixed at
stage 40. Development of the pronephros was analyzed by staining with the
monoclonal antibody 3G8. (B) Pronephric tubule formation was inhibited in XTRAP-
γ MO-injected embryos (arrow in B) compared with the non-injected-side (arrow
in C) and the embryos injected 5-mis-XTRAP-γ MO (arrow in D). The phenotype of
the pronephric tubules was rescued by co-injection of XTRAP-γ MO with 5-mis-
XTRAP-γ mRNA (E). (F,G) Histological analysis of XTRAP-γ MO injected embryo
and 5-mis-XTRAP-γ MO injected embryos. Pronephros tubules in XTRAP-γ MOinjected
embryos showed severe developmental defects (arrow in F) compared
with that of the 5-mis-XTRAP-γ MO-injected embryos (arrow in G). Image published in: Li DH et al. (2005) Copyright © 2005. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View |