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XB-IMG-83459

Xenbase Image ID: 83459


Fig. 5. Whole-mount in situ hybridization shows the effect on expression of pronephros marker genes XTRAP-γ MO injection. Embryos were injected with XTRAP- γ MO (40 ng) or 5-mis-XTRAP-γ MO (40 ng) and synthetic of β-galactosidase mRNA (250 ng), then fixed at stage 30/31 (A-P) and stage 22/23 (Q-T). Expression of Lim-1 was increased in the side of embryos injected with XTRAP-γ MO (A), compared the non-injected side (B). Expression of Pax-2 and Wnt-4 was inhibited by injection of XTRAP- γ MO (E,I), compared with noninjected side (F,J). For those embryos fixed at stage 22/23, expression of Pax-2 (Q) and Wnt-4 (S) in embryos fixed at stage 22/23 was not affected by injection of XTRAP- γ MO or the control 5-mis-XTRAP- γ MO (R,T). Expression of Pax-8 was not affected by XTRAP-γ MO (M,N). The expression of Lim-1 (C,D), Pax-2 (G,H), Wnt-4 (K,L) and Pax-8 (O,P) was unchanged in embryos injected with 5-mis- XTRAP-γ MO.

Image published in: Li DH et al. (2005)

Copyright © 2005. Reproduced with permission of the Publisher, University of the Basque Country Press.

GeneSynonymsSpeciesStage(s)Tissue
lhx1.LXlim1, Xlim-1, Lim1, lim-1, lim1X.laevisThroughout NF stage 29 and 30pronephric mesenchyme
brain
midbrain
hindbrain
anterior dorsal lateral plate region
pax2.LXPax2, XPax-2, pax-2, pax2-a, pax2-bX.laevisThroughout NF stage 29 and 30 to NF stage 31anterior dorsal lateral plate region
brain
hindbrain
post-anal gut
optic stalk
visceral pouch
central nervous system
spinal cord
wnt4.LXwnt4, Xwnt-4, wnt-4X.laevisThroughout NF stage 29 and 30 to NF stage 31pronephric mesenchyme
anterior dorsal lateral plate region
pharyngeal arch
neural tube
spinal cord
pax8.LXPax8, XPax-8, pax-8X.laevisThroughout NF stage 29 and 30 to NF stage 31otic vesicle
pronephric mesenchyme
anterior dorsal lateral plate region
pax2.LXPax2, XPax-2, pax-2, pax2-a, pax2-bX.laevisThroughout NF stage 22 to NF stage 23intermediate mesoderm
neural tube
wnt4.LXwnt4, Xwnt-4, wnt-4X.laevisSometime during NF stage 22 to NF stage 23intermediate mesoderm
neural tube

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