XB-IMG-83462
Xenbase Image ID: 83462
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Figure 1. An RNAi Screen Identified RAB8B as a Positive Regulator of Wnt/β-catenin Pathway
(A) RAB8B ranked the top-scoring candidate in the Rab GTPase family. Heatmap displaying individual siRNA Z scores in HCT116.
(B) Wnt reporter assay showing percent of control (POC) normalized relative luminescence unit (RLU) screen scores of RAB8B siRNAs, and their confirmation in HCT116.
(C) Human RAB8B-RNAi-insensitive expression construct, harboring silent mutations in the siRNA#2 complementary region, rescued siRNA#2 phenotype but failed to rescue the pool that also contains targeting siRNAs #1 and #3 (HEK 293T). Image published in: Demir K et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |