XB-IMG-83463
Xenbase Image ID: 83463
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Figure 2. RAB8B�s Function Was Required at the Level of Receptors in the Wnt-Receiving Cell
(A) RAB8B depletion reduced Wnt reporter activity when activated with Wnt3a conditioned medium (Wnt3a CM) and Dvl1 but not constitutively active LRP6Δe1-4 coexpression in HEK 293T cells.
(B) RAB8B knockdown reduced Wnt3a-induced active LRP6 (Sp1490/GSK3β phosphorylation site) and β-catenin levels, whereas total protein levels remained unchanged in HEK 293T cells.
(C) RAB8B depletion reduced signalosome formation as detected by lower LRP6 phosphorylation (Tp1479/CK1γ) after 1.5 hr of Wnt3a exposure in HEK 293T. Image published in: Demir K et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |