XB-IMG-83472
Xenbase Image ID: 83472
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Figure 1. Importance of H3.2 and H3.3 during Early Development
(A) Specificity of H3.3 and H3 morpholinos. (Left) The scheme shows injections of indicated morpholinos (MO) in fertilized eggs (13.8 ng). After incubation to reach stage 12 in controls, 80 embryos were used to prepare soluble fractions for western blot analysis. (Right) Results used a 2-fold dilution series (gradient bar) with the highest quantity being equivalent to three embryos, and detection with indicated antibodies (see also Figure S2A). Anti-β actin and memcode staining served as loading controls.
(B) H3.3 and H3 downregulation leads to distinct phenotypes. The scheme shows injections of indicated MO (4.6 ng) in one cell of 2-cell stage embryos. We kept embryos at 18�C and acquired images at the indicated stages (using control embryos as a reference). White arrows point to the gastrulation defects in H3.3 morphants. In the bottom panel, we show a broader coverage of the gastrulation time period (stages 11�13) with eight distinct time points (see also Movie S1). Scale bar: 1 mm.
See also Figures S1, S2, and S3. Image published in: Szenker E et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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