Xenbase Image ID: 83479

Figure S2. Specificity of H3.2 and H3.3 Antibodies and Histone Chaperones Phenotypes during Early Development, Related to Figures 1 and 2 (A) Specificity of H3.2 and H3.3 antibodies. We used wheat germ extracts to in vitro translate H3.2-HA and H3.3-HA mRNAs and analyzed them by Western blot using specific antibodies as indicated. Asterisks (∗) show unspecific bands also present in extracts without exogenous mRNA (−) while the arrow indicates the H3-HA band (H3.2-HA or H3.3-HA). Memcode staining served as a loading control. (B) HIRA phenotype phenocopies H3.3 phenotype while MO p150 phenocopies the one of MO H3 injected embryos. We injected indicated MO (13.8ng for H3 and H3.3, 138ng for CTL, p150 and HIRA MO) in one cell of two-cell stage embryos. We show the gastrulation time periode between stages 11 and 13 with 8 distinct time points (see also Figures 1 and 5, and Movie S1). Scale bar: 1 mm. Image published in: Szenker E et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + chaf1a MO + NF11-13 (morphology) fig.S2.b Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore Xla Wt + h3f3a MO + NF11-13 (morphology) fig.S2.b Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore Xla Wt + hira MO + NF11-13 (morphology) fig.S2.b Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore Xla Wt + hist1h3g MO + NF11-13 (morphology) fig.S2.b Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore Larger Image Printer Friendly View

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