Xenbase Image ID: 83480

Figure S3. Dose-Dependent Phenotypes and Chaperone Expression throughout Development, Related to Figures 1 and 2 (A) Dose-dependent phenotype of H3.3 and HIRA morpholinos. We injected CTL, H3.3 or HIRA MO (at the indicated doses) in one cell of two-cell stage embryos and acquired images when control embryos reached stage 28. Scale bar: 1 mm. (B) Expression of histone chaperones during early development. Embryos at indicated stages were used to prepare soluble fractions and were analyzed by Western blot. We used a 2-fold dilution series as indicated (gradient bar) with the highest quantity being equivalent to 3 embryos, and revealed with specific antibodies as indicated. βactin served as a loading control. Asterisk (∗) shows an unspecific band, sketches are adapted from (Nieuwkoop and Faber, 1967). (C) Control injection phenotypes. We injected the indicated MO (4.6 ng of mutH3 and mutH3.3 MO, 46 ng of CTL and mutHIRA MO) in one cell of two-cell stage embryos and acquired images when control embryos reached stage 28. Scale bar: 1 mm. Image published in: Szenker E et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + h3f3a MO + NF28 (morphology) fig.S3.a Anatomical Phenotype abnormal anterior-posterior axis morphology decreased length of anterior-posterior axis decreased size of the head Xla Wt + h3f3a MO + NF28 (morphology) fig.S3.a Anatomical Phenotype abnormal anterior-posterior axis morphology decreased length of anterior-posterior axis decreased size of the head Xla Wt + h3f3a MO + NF28 (morphology) fig.S3.a Anatomical Phenotype abnormal bending of anterior-posterior axis decreased length of anterior-posterior axis decreased size of the head Xla Wt + hira MO + NF28 (morphology) fig.S3.a Anatomical Phenotype abnormal anterior-posterior axis, curved dorsal decreased length of anterior-posterior axis decreased size of the head decreased size of the tail Xla Wt + hira MO + NF28 (morphology) fig.S3.a Anatomical Phenotype abnormal anterior-posterior axis, curved lateral decreased size of the head decreased size of the tail Xla Wt + hira MO + NF28 (morphology) fig.S3.a Anatomical Phenotype decreased length of anterior-posterior axis decreased size of the head decreased size of the tail Larger Image Printer Friendly View

Return to previous page