Xenbase Image ID: 83516

Fig. 5. Treatment with retinoic acid antagonist alters the expression domains of Nkx2.5 and GATA-4. The expression domains of Nkx2.5 and GATA-4 are visualized by whole-mount in situ hybridization. In control embryos treated with 2 μl/ml DMSO (top panel, A–D), the normal expression domain of Nkx2.5 and GATA-4 can be seen in side view and viewed from the ventral side. Treatment with 1 μM RA caused a severe reduction in the expression pattern of Nkx2.5 (E, F) but did not cause any obvious change in the expression pattern of GATA-4 (G, H). Treatment with 1 μM AGN 194301 caused a marked change in the expression domain of Nkx2.5. As expected, the expression domain was restricted to a sheet of cells rather than a tube, and the expression was restricted to the posterior end of the region that normally expresses cTnI (I, J). In addition, there was a marked spur of Nkx2.5 expression that extended dorsally close to the level of the somites (red arrow). Treatment with AGN 194301 caused a reduction, but not elimination of the GATA-4 expression domain (K, L). If embryos were simultaneously exposed to 1 μM RA and 1 μM AGN 194301, the expression domains of Nkx2.5 and GATA-4 were similar to controls (M–P). Image published in: Collop AH et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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