Xenbase Image ID: 83517

Fig. 6. Treatment of embryos with antagonist prior to gastrulation alters patterning of cardiac genes. At embryonic stage 20, Nkx2.5 is normally expressed in a broad sheet on either side of the cement gland (A). When embryos were treated with AGN 194301 prior to gastrulation (stage 6), this domain appeared expanded, particularly along the anterior–posterior axis (B). When compared to expression in control embryos (C), the expression of the lateral plate mesoderm marker, HAND1, was diminished by the same treatment (D) with shortening both from the posterior and anterior end of the embryo (anterior is left and posterior is right in both C and D). Despite the early changes in Nkx2.5 expression, the myocardium still failed to form a tube and the Nkx2.5 (E) and cTnI (F) expression domain was reduced with essentially the same phenotype as observed in embryos treated with RA antagonists post-gastrulation (see Fig. 1). Image published in: Collop AH et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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