Xenbase Image ID: 83593

Fig. 1. Localization of a neural-specific mRNA by whole-mount in situ hybridization. (A) Late blastula (stage 9; Nieuwkoop and Faber. 1967). The signal is found in the dorsal (O) and the ventral (V) ectoderm. AP animal pole, VP vegetal pole. (8) Mid-gastrula (stage 11 1/2, large yolk plug; lateral view) The signal can be identified on the dorsal side (O) and also in the ventral (V, presumptive epidermis) and lateral parts (YP yolk plug). (C) Late gastrula (stage 12; dorsal view). The presumptive neural plate is intensively stained (left side: cranial, right side: caudal). (DJ Early neurula (stage 13; lateral view). The signal is now found mainly in the neural plate. (E) Late neural fold stage (stage 17. dorsal view). Only the presumptive brain area and the presumptive spinal cord are stained. (F, G) Initial neural tube stage (stage 19. F dorsal view. G view on the furure head area). The signal is restricted to the presumptive brain and spinal cord (SP) zone. A/so the presumptive cement gland (CE) /s stained. (H, J) Larval stage (stage 37; H lareral view, J dorsal view). The distribution of the transcript can be found In the brain and the spinal cord. Note that also the optic nerve (OP) is stained. (KJ Advanced larva (stage 43, dorsal view). The staining is detected in the brain. the spinal cord and a/so clearly in the optic nerve (arrow). Image published in: Oschwald R et al. (1991) Copyright © 1991. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 9 ectoderm tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 11.5 to NF stage 12 ectoderm non-neural ectoderm neuroectoderm tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 13 neural plate pre-chordal neural plate chordal neural plate tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 17 neural fold tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 19 brain spinal cord cement gland primordium neural tube optic field eye primordium tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 37 and 38 brain spinal cord optic nerve cephalic nerve forebrain midbrain hindbrain tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 43 brain spinal cord optic nerve cephalic nerve Image source: Published Larger Image Printer Friendly View

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