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Fig. 3. Demonstration of the neural-specific expression of the 13- tubulin gene (clone 24-1 0). RNA preparations from different adult tissues were compared. 10,ugof RNA was applied on each lane. (A) The N'rTRANEmembrane was washed 2 times with 2x SSPE at 6S:C. The film was exposed for 2 days. IB) The same membrane as In (A) was washed again with 0.2 x SSPE (high stnngencv) at 6fY'C for 20 min followed by a film exposure for 3 days. The stringency in Fig. 38 corresponds to that used in the whole-mount In Situ preparations. B, brain; K, kidney; L. liver; Lu, lung; M, muscle; S. skin

Image published in: Oschwald R et al. (1991)

Copyright © 1991. Reproduced with permission of the Publisher, University of the Basque Country Press.

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