XB-IMG-83609
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Figure S2. PACSIN2 knock down delay neurulation
(A) The PACSIN2 double morphant delay neurulation. Embryos were injected at the 2-
cell stage in both blastomeres with 80 ng of either the control morpholino (MO Ctl) or the
mix of AS morpholino against PACSIN2 and 2� (MO2+2�). The embryonic development
was carefully monitored until tail bud stage and a picture of representative embryo was
taken at neurula (dorsal view, anterior up) and tailbud stage (lateral view, anterior left and
dorsal up). No differences were noted during gastrulation between embryos injected with
MO Ctl and MO2+2�. When control embryos reached mid-neurula stage, each embryo
was scored for its exact stage according to the Nieuwkoop and Faber Table. The
histogram represents the distribution of embryos at different neurula stage of a
representative experiment. The number of embryos analyzed was as follow: MO Ctl= 39;
MO 2+2�=36. During neurulation, MO2+2� injected embryos displayed a delay in
neurulation. However, embryos eventually closed their neural tube and appeared to
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continue developing normally. (B) The PACSIN2 morpholino does not prevent neural
induction. Dorsal view of embryos treated by whole mount hybridization at stage 18 and
22 with the neural marker N-tubulin. Anterior is up. When control embryo reached stage
18, embryos injected with MO2+2� appeared to be at a younger stage. The N-tubulin
pattern indicates that neural tissue is present. At stage 22, embryos injected with MO2+2�
have close the neural tube and express N-tubulin in the two dorsal rows of neurons in the
neural tubes like the controls (white arrows). Unlike control embryos, groups of Ntubulin
positive cells are seen outside of the neural tube in the morphant embryos
(arrowheads), indicating that those neurons failed to move dorsally. This suggests that
PACSIN2 could be involved in cell movement during neurulation. Image published in: Cousin H et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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