Xenbase Image ID: 83638

Figure 3. Glycosylation not required for protein stability or Nodal-blocking function. A:Xenopus oocytes were injected with RNA encoding Xlefty or either non-glycosylatable mutant, XleftyNGM-A or XleftyNGM-S. Arrowheads, XleftyL and XleftyS. B: Western blot, conditioned medium from animal halves from embryos injected at the one-cell stage with 100, 500, or 1,500 pg of Xleftymyc or XleftyNGM-Amyc RNA. Filled arrowheads, proprotein and XleftyL; open arrowhead, XleftyS. C: Mesoderm induction assay for effectiveness of non-glycosylated Xlefty. Marker expression was assayed at stage 10.5 in animal caps explanted from embryos injected with Xnr2 (pg/embryo indicated) with or without RNA encoding Xlefty, XleftyNGM-A, Xleftymyc, or XleftyNGM-Amyc. Un, uninjected oocyte or embryo; Vehicle, 1% DMSO; (−) conditioned medium from oocytes not injected with tunicamycin; T, tunicamycin; L, XleftyL; S, XleftyS; P, proprotein; WE, whole embryo plus (+) and minus (−) reverse transcriptase (RT); AC, uninjected animal cap. 1:1, 10 pg of each RNA; 10:1, 100 pg of RNA encoding untagged or tagged versions of Xlefty or XleftyNGM-A and 10 pg of RNA encoding Xnr2.Download figure to PowerPoint Image published in: Westmoreland JJ et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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