Xenbase Image ID: 83639

Figure 4. Range of Xlefty movement is not altered by glycosylation. A–H: Embryos co-injected with 250 pg of LacZ RNA plus or minus 150 pg of tagged or untagged RNAs encoding Xlefty and XleftyNGM-A and stained for Red-Gal and then Myc-immunostained. A–D: Dashed line, boundary of Red-Gal lineage-label-marked producer cell clone. Broken arrow, direction of vegetal pole. Specific Myc immunodetection signal is purple (arrowhead). Embryos were visualized externally after staining in whole-mount. Embryos were uninjected (A), or received 150 pg untagged Xlefty RNA (B), 150 pg Xleftymyc RNA (C), or 150 pg XleftyNGM-Amyc RNA (D). E–H: Embryos were bisected through the patch of Red-Gal staining before myc immunostaining. Embryos were uninjected (E) or received 150 pg untagged Xlefty RNA (F), 150 pg XleftyMyc RNA (G), or 150 pg XleftyNGM-Amyc RNA (H). Image published in: Westmoreland JJ et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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