XB-IMG-83668
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Fig. 4. Expression of vimentin in stage-19, -22 and -24 embryos. (Note: the diagram in this and Figs 5, 6, 7 and 8 indicates the optical section planes presented.) In an anterior-dorsal view of a stage-19 embryo stained with RV202 (A) the neural tube (nt) runs vertically down the centre of the embryo and ends with two bulbs that are the nascent optic vesicles (ov). Vimentin-expressing cells line the sides of the neural tube (nv). Individual round or wedge-shaped cells can be identified through the cleared ectoderm and neural tube. There does not appear to be any staining of the optic vesicles themselves. In an anterior view of a stage-22 embryo stained with RV202 (B), the outer lateral margin of the neural tube contains many cells that are darkly stained for vimentin (nv). There is no staining for vimentin in the optic vesicles. Staining of the cement gland (c) is artifactual. Vimentin-positive cells (nv) can be seen in a lateral view of the optic vesicle of a stage-24 embryo stained with 14h7 (C). Dorsal (dor) is left and ventral (ven) is right in C. Dorsal is at the top and ventral is at the bottom in B. Bars in A and B, 100^m; in C, 250um Image published in: Dent JA et al. (1989) Copyright © 1989. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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