Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-IMG-83701

Xenbase Image ID: 83701


Figure 3. Long-Range Feedback for Expression of chordin and sizzled (A-C) qPCR analysis of transcriptional control for sizzled and bmp4 (A, ventral), myoD (B, lateral), and chordin, admp, and shh (C, dorsal) in the β-catenin-MO-injected embryo at stage 12 (n = 6; three experiments). The values in qPCR (per embryo) were normalized with the internal control (luciferase mRNA) included at the reaction (see Experimental Procedures). The expression levels of the wild-type embryo (Cont; no MO injection) was defined as 1. Increasing amounts of Noggin were injected into the blastocoel of the late blastula as in Figure 2A. Bars represent SD. (D-K) Expression of sizzled in embryos at stages 10 (D and H), 11 (E and I), 12 (F and J), and 13 (G and K). (D) through (G) show control embryos, and (H) through (K) show chordin-MO-injected embryos. (L-S) Expression of chordin in embryos at stages 10 (L and P), 11 (M and Q), 12 (N and R), and 13 (O and S). (L) through (O) show control embryos, and (P) through (S) show sizzled-MO-injected embryos. (T and U) Quantification of expression levels of sizzled (T) and chordin (U) by qPCR (n = 8; three experiments). Bars represent SD. (V) Schematic of the feedback loop involving Chordin, BMP, Sizzled, and Chordin proteinases. (W) Simplified view of the axis-wide long-range feedback by Chordin and Sizzled.

Image published in: Inomata H et al. (2013)

Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V.

GeneCloneSynonymsSpeciesStage(s)Tissue
szl.L secreted frizzled, xszl, sizzled, frzb3X.laevisThroughout NF stage 10marginal zone
ventral marginal zone
ventro-lateral marginal zone
dorso-lateral marginal zone
szl.L secreted frizzled, xszl, sizzled, frzb3X.laevisThroughout NF stage 11involuted ventral mesoderm
szl.L secreted frizzled, xszl, sizzled, frzb3X.laevisThroughout NF stage 12involuted ventral mesoderm
szl.L secreted frizzled, xszl, sizzled, frzb3X.laevisThroughout NF stage 13involuted ventral mesoderm
chrd.1.S chd, chordin, X-chordin, chrdX.laevisThroughout NF stage 10.5upper blastopore lip
chrd.1.S chd, chordin, X-chordin, chrdX.laevisThroughout NF stage 11upper blastopore lip
involuted dorsal mesoderm
chrd.1.S chd, chordin, X-chordin, chrdX.laevisThroughout NF stage 12presumptive axial mesoderm
chrd.1.S chd, chordin, X-chordin, chrdX.laevisThroughout NF stage 13notochord

Image source: Published

Larger Image
Printer Friendly View

Quality:
Good quality Poor quality
comment [+]
Return to previous page
Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.11.3


Major funding for Xenbase is provided by grant P41 HD064556