XB-IMG-83704
Xenbase Image ID: 83704
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Figure S6.
Computer Simulation of DV Patterning Based on the "Long-Range Accumulation and Feedback" Model, Related to Figure 6
(A) Space-time plots of regional production of Chordin, ADMP, Sizzled, and BMP4.
(B) Space-time plots of protein distribution of Chordin (total), Sizzled, free BMP4, free ADMP, and free BMPs (free-ADMP + free-BMP4).
(C�L) Simulation results at 20 hr. (C) Temporal profiles of regional production of Chordin, ADMP, Sizzled and BMP4. (D) Simulated protein distribution of free BMP4 and free ADMP. (E) Simulated protein distribution of BMP4-Chordin and ADMP-Chordin complexes. (F) Simulation results showing embryo-size-dependent changes of average Sizzled concentrations in the wild-type and ADMP-depleted embryos. (G) Embryo-size-dependent distribution of total Chordin, free BMPs, and Sizzled proteins along the DV axis. The y-axis stands for protein levels for Sizzled, total Chordin (left scale), and free BMPs (right scale). (H) Collapse of scaling upon a further body-size reduction in the simulation. The quarter-sized embryo (around the dorsal end) became hyperdorsalized in (I) (no scaling) and expressed little ventral marker in (J) and (K) (no sizzled expression in 100%, n = 16). In (L), scaling occurred, but within a narrower range, in the simulated ADMP-depleted embryo.
(M) ADMP-depletion caused a moderate dorsalization in the embryo.
(N) Bisection caused a strong hyperdorsalization (see a catastrophic expansion of shh expression) in the ADMP-depleted embryo, unlike the wild-type (Figure 5G).
(O) Simulated effects of embryo-size reduction on relative DV patterning in the wild-type embryo at 20, 50, and 100 hr. Image published in: Inomata H et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |